Open AccessAdvancing Solutions to the Carbohydrate Sequencing Challenge
Author(s) -
Christopher Gray,
Lukasz Migas,
Perdita E. Barran,
Kevin Pagel,
Peter H. Seeberger,
Patrick A. Eyers,
GeertJan Boons,
Nicola L. B. Pohl,
Isabelle Compag,
Göran Widmalm,
Sabine L. Flitsch
Publication year - 2019
Publication title -
journal of the american chemical society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.115
H-Index - 612
eISSN - 1520-5126
pISSN - 0002-7863
DOI - 10.1021/jacs.9b06406
Subject(s) - chemistry , glycosidic bond , monosaccharide , carbohydrate , computational biology , nuclear magnetic resonance spectroscopy , carbohydrate chemistry , combinatorial chemistry , biochemical engineering , stereochemistry , organic chemistry , engineering , biology , enzyme
Carbohydrates possess a variety of distinct features with stereochemistry playing a particularly important role in distinguishing their structure and function. Monosaccharide building blocks are defined by a high density of chiral centers. Additionally, the anomericity and regiochemistry of the glycosidic linkages carry important biological information. Any carbohydrate-sequencing method needs to be precise in determining all aspects of this stereodiversity. Recently, several advances have been made in developing fast and precise analytical techniques that have the potential to address the stereochemical complexity of carbohydrates. This perspective seeks to provide an overview of some of these emerging techniques, focusing on those that are based on NMR and MS-hybridized technologies including ion mobility spectrometry and IR spectroscopy.