Open AccessTransmembrane Fluoride Transport: Direct Measurement and Selectivity Studies
Author(s) -
Harriet J. Clarke,
Ethan N.W. Howe,
Xin Wu,
Fabian Sommer,
Masafumi Yano,
Mark E. Light,
Stefan Kubik,
Philip A. Gale
Publication year - 2016
Publication title -
journal of the american chemical society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.115
H-Index - 612
eISSN - 1520-5126
pISSN - 0002-7863
DOI - 10.1021/jacs.6b10694
Subject(s) - chemistry , valinomycin , fluoride , ion transporter , selectivity , membrane transport , ionophore , transmembrane protein , transporter , membrane , monensin , biophysics , inorganic chemistry , biochemistry , receptor , biology , gene , catalysis
Fluoride has been overlooked as a target in the development of synthetic anion transporters despite natural fluoride transport channels being recently discovered. In this paper we report the direct measurement of fluoride transport across lipid bilayers facilitated by a series of strapped calix[4]pyrroles and show that these compounds facilitate transport via an electrogenic mechanism (determined using valinomycin and monensin coupled transport assays and an additional osmotic response assay). An HPTS transport assay was used to quantify this electrogenic process and assess the interference of naturally occurring fatty acids with the transport process and Cl - over H + /OH - transport selectivity.