Open AccessReduction TriggeredIn SituPolymerization in Living Mice
Author(s) -
Lina Cui,
Sandro Vivona,
Bryan Smith,
Sri-Rajasekhar Kothapalli,
Jun Li,
Xiaowei Ma,
Zixin Chen,
Madelynn Taylor,
Paul H. Kierstead,
Jean M. J. Fréchet,
Sanjiv S. Gambhir,
Jianghong Rao
Publication year - 2020
Publication title -
journal of the american chemical society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.115
H-Index - 612
eISSN - 1520-5126
pISSN - 0002-7863
DOI - 10.1021/jacs.0c07594
Subject(s) - chemistry , polymerization , in situ , fluorophore , in situ polymerization , biophysics , scaffold , small molecule , linker , polymer , in vivo , fluorescence , combinatorial chemistry , nanotechnology , photochemistry , organic chemistry , biochemistry , biomedical engineering , biology , medicine , physics , materials science , microbiology and biotechnology , quantum mechanics , computer science , operating system
"Smart" biomaterials that are responsive to physiological or biochemical stimuli have found many biomedical applications for tissue engineering, therapeutics, and molecular imaging. In this work, we describe in situ polymerization of activatable biorthogonal small molecules in response to a reducing environment change in vivo . We designed a carbohydrate linker- and cyanobenzothiazole-cysteine condensation reaction-based small molecule scaffold that can undergo rapid condensation reaction upon physiochemical changes (such as a reducing environment) to form polymers (pseudopolysaccharide). The fluorescent and photoacoustic properties of a fluorophore-tagged condensation scaffold before and after the transformation have been examined with a dual-modality optical imaging method. These results confirmed the in situ polymerization of this probe after both local and systemic administration in living mice.