Tet-Mediated Formation of 5-Hydroxymethylcytosine in RNA | Zendy

Lijuan Fu | Zendy; Candace R. Guerrero | Zendy; Na Zhong | Zendy; Nicholas J. Amato | Zendy; Yunhua Liu | Zendy; Shuo Liu | Zendy; Qian Cai | Zendy; Debin Ji | Zendy; SeungGi Jin | Zendy; Laura J. Niedernhofer | Zendy; Gerd P. Pfeifer | Zendy; Guo-Liang Xu | Zendy; Yinsheng Wang | Zendy

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Tet-Mediated Formation of 5-Hydroxymethylcytosine in RNA

Author(s) -

Lijuan Fu,

Candace R. Guerrero,

Na Zhong,

Nicholas J. Amato,

Yunhua Liu,

Shuo Liu,

Qian Cai,

Debin Ji,

SeungGi Jin,

Laura J. Niedernhofer,

Gerd P. Pfeifer,

Guo-Liang Xu,

Yinsheng Wang

Publication year - 2014

Publication title -

journal of the american chemical society

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 7.115

H-Index - 612

eISSN - 1520-5126

pISSN - 0002-7863

DOI - 10.1021/ja505305z

Subject(s) - 5 hydroxymethylcytosine , chemistry , rna , enzyme , epigenetics , dna , in vitro , biochemistry , messenger rna , chromosomal translocation , 5 methylcytosine , dna methylation , gene , gene expression

Oxidation of 5-methylcytosine in DNA by ten-eleven translocation (Tet) family of enzymes has been demonstrated to play a significant role in epigenetic regulation in mammals. We found that Tet enzymes also possess the activity of catalyzing the formation of 5-hydroxymethylcytidine (5-hmrC) in RNA in vitro. In addition, the catalytic domains of all three Tet enzymes as well as full-length Tet3 could induce the formation of 5-hmrC in human cells. Moreover, 5-hmrC was present at appreciable levels (∼1 per 5000 5-methylcytidine) in RNA of mammalian cells and tissues. Our results suggest the involvement of this oxidation in RNA biology.

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