Open AccessDetection of Homocysteine and Cysteine
Author(s) -
Weihua Wang,
Oleksandr Rusin,
Xiangyang Xu,
Kyu Kwang Kim,
Jorge O. Escobedo,
Sayo O. Fakayode,
Kristin A. Fletcher,
Mark Lowry,
Corin M. Schowalter,
Candace M. Lawrence,
Frank R. Fronczek,
Isiah M. Warner,
Robert M. Strongin
Publication year - 2005
Publication title -
journal of the american chemical society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.115
H-Index - 612
eISSN - 1520-5126
pISSN - 0002-7863
DOI - 10.1021/ja054962n
Subject(s) - chemistry , cysteine , homocysteine , neurotoxicity , biochemistry , toxicity , organic chemistry , enzyme
At elevated levels, homocysteine (Hcy, 1) is a risk factor for cardiovascular diseases, Alzheimer's disease, neural tube defects, and osteoporosis. Both 1 and cysteine (Cys, 3) are linked to neurotoxicity. The biochemical mechanisms by which 1 and 3 are involved in disease states are relatively unclear. Herein, we describe simple methods for detecting either Hcy or Cys in the visible spectral region with the highest selectivity reported to date without using biochemical techniques or preparative separations. Simple methods and readily available reagents allow for the detection of Cys and Hcy in the range of their physiologically relevant levels. New HPLC postcolumn detection methods for biological thiols are reported. The potential biomedical relevance of the chemical mechanisms involved in the detection of 1 is described.