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Transfection of Cells Mediated by Biodegradable Polymer Materials with Surface‐Bound Polyethyleneimine
Author(s) -
Zheng Ji,
Manuel William S.,
Hornsby Peter J.
Publication year - 2000
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp990150h
Subject(s) - transfection , polymer , covalent bond , glycolic acid , lactic acid , chemistry , polymer chemistry , amino acid , materials science , surface modification , chemical engineering , organic chemistry , biochemistry , bacteria , gene , genetics , biology , engineering
Poly(ϵ‐CBZ‐ L ‐lysine) can be mixed with biodegradable polymers such as poly( D , L ‐lactic‐ co ‐glycolic acid) or poly( L ‐lactic acid) and formed into films, foams, or microspheres. Surface amino groups may then be deprotected with acid or lithium/liquid ammonia. The amino groups serve as a method to modify the surface by attachment of other molecules. In the present experiments, we show that these polymer materials, as films or foams, may be surface modified by the attachment of polyethyleneimine (PEI). Plasmid DNA attached to the PEI can transfect cells plated on the surface over several days. Covalent atachment of PEI was required for transfection to be efficient. PEI was also attached to surface‐bound collagen on cell culture plates and was shown to mediate transfection.