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Proteomics: Theoretical and Experimental Considerations
Author(s) -
Hatzimanikatis Vassily,
Choe Leila H.,
Lee Kelvin H.
Publication year - 1999
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp990004b
Subject(s) - proteome , decipher , computational biology , proteomics , function (biology) , biology , genome , organism , translation (biology) , computer science , transcription (linguistics) , protein expression , data science , gene , messenger rna , bioinformatics , genetics , linguistics , philosophy
Cellular engineering relies on the ability to decipher the genetic basis of various phenotypes. Emerging technologies for analyzing the biological function of the information encoded in the genome of particular organisms and/or tissues focus on the monitoring of transcription (mRNA) and translation (protein) processes. Elementary theoretical considerations presented in this article strongly suggest that a combination of mRNA and protein expression patterns should be simultaneously considered to fully develop a conceptual understanding of the functional architecture of genomes and gene networks. We propose a framework of experimental and mathematical methods for acquiring and analyzing quantitative proteomic information and discuss recent developments in proteome analytical technology.