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Role of Physical Properties of Glycidyl Methacrylate− Pentaerythritol Triacrylate Copolymers on Immobilization of Milk Clotting Protease from Aspergillus niger MC4
Author(s) -
Channe Prakash S.,
Shewale Jaiprakash G.,
Ghadge Varsha B.,
Raman Rajan C.,
Ponrathnam Surendra
Publication year - 1998
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp9800900
Subject(s) - protease , aspergillus niger , glycidyl methacrylate , chemistry , methacrylate , immobilized enzyme , clotting time , pentaerythritol , chromatography , copolymer , enzyme , polymer chemistry , nuclear chemistry , polymer , organic chemistry , biochemistry , coagulation , psychology , fire retardant , psychiatry
Abstract The effect of cross‐link density and amount of porogen on physical properties of the glycidyl methacrylate−pentaerythritol triacrylate (GMA−PETA) copolymer and intern on the immobilization of milk clotting protease from Aspergillus niger MC4 was studied. Almost quantitative expression of the milk clotting protease was achieved with copolymer of 251% cross‐link density and generated with 65.4 mL of cyclohexanol as porogen. The pH of 4.0 and 18 h period were the best conditions for binding the milk clotting protease onto the polymer. The immobilized milk clotting protease prepared had an activity of 6000 units/g. The optimum pH and temperature ranges for both soluble and immobilized milk clotting protease preparations were pH 5.6–5.8 and 55–60 °C, respectively. Immobilized milk clotting protease was more stable than the soluble enzyme; the half‐lives at pH 4.0 and 37 °C were 160 and 60 days, respectively. At pH 5.8 and 37 °C, the parameters favorable for milk clotting, the immobilized enzyme preparation did not exhibit any aspartic protease activity.