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Intermittent Light Irradiation with a Second‐Scale Interval Enhances Caffeine Production by Coffea arabica Cells
Author(s) -
Kurata Hiroyuki,
Achioku Tomohisa,
Okuda Naoyuki,
Furusaki Shintaro
Publication year - 1998
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp980065u
Subject(s) - coffea arabica , caffeine , photobioreactor , darkness , irradiation , light intensity , horticulture , biology , botany , chemistry , food science , physics , optics , agronomy , biomass (ecology) , nuclear physics , endocrinology
We developed novel equipment that intermittently illuminates Coffea arabica cell suspensions at a second‐scale interval and investigated how intermittent irradiation enhances caffeine biosynthesis by C. arabica cells. The light/dark cycles consisting of 2 s of illumination and 18 s of darkness enhanced caffeine production, reaching the same level as for continuous light. The intermittent illumination increased the production efficiency regarding light consumption by a factor of 10. Caffeine production was determined by light intensity regardless of intermittent or continuous light irradiation. We propose a new concept for designing a photobioreactor that is applicable to secondary metabolite production by plant cell culture.