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Protein Adsorption Characteristics of a Sulfonic‐Acid‐Group‐Containing Nonwoven Fabric
Author(s) -
Kim Min,
Sasaki Masami,
Saito Kyoichi,
Sugita Kazuyuki,
Sugo Takanobu
Publication year - 1998
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp980060x
Subject(s) - lysozyme , glycidyl methacrylate , monomer , nonwoven fabric , sulfonic acid , adsorption , chemistry , polymer chemistry , polymer , chemical engineering , methacrylate , group (periodic table) , organic chemistry , fiber , biochemistry , engineering
A sulfonic acid (SO 3 H)‐group‐containing nonwoven fabric was prepared by radiation‐induced graft polymerizaton of an epoxy‐group‐containing monomer, glycidyl methacrylate, onto a nonwoven fabric and subsequent sulfonation. The nonwoven fabric containing SO 3 H groups at different densities was equilibrated with lysozyme in a buffer (pH 6.0). The equilibrium binding capacity (EBC) of lysozyme increased linearly with increasing SO 3 H group density. The SO 3 H‐group‐containing polymer chains extended from the pore surface due to mutual electrostatic repulsion and held lysozyme in multilayers. The maximum EBC was 0.7 kg/kg at a SO 3 H group density of 1.7 mol/kg.
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