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Asymmetric Reduction of Acetophenone with Calcium‐Alginate‐Entrapped Baker's Yeast in Organic Solvents
Author(s) -
Griffin Daniel R.,
Yang Fangxiao,
Carta Giorgio,
Gainer John L.
Publication year - 1998
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp9800586
Subject(s) - acetophenone , cofactor , chemistry , calcium alginate , yeast , enantiomeric excess , alcohol , fermentation , enantiomer , ethanol , catalysis , organic chemistry , chromatography , enantioselective synthesis , biochemistry , enzyme , calcium
Baker's yeast cells entrapped in alginate beads are shown to catalyze reactions in organic solvents when a cofactor regeneration scheme is implemented. This study focused on the reduction of acetophenone to 1‐phenylethanol, using baker's yeast as well as a cosubstrate to regenerate the cofactor. The product is a chiral alcohol, and it was desired to maintain a high enantiomeric excess. The effects of parameters such as the addition of a cosubstrate, water content, fermentation time, buffer pH, and bead diameter have been investigated. Such a general process may be quite useful when single enantiomers are needed, as well as for the production of other chemicals.