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Stabilization of Active Recombinant Retroviruses in an Amorphous Dry State with Trehalose
Author(s) -
Bieganski Robert M.,
Fowler Alex,
Morgan Jeffrey R.,
Toner Mehmet
Publication year - 1998
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp980057d
Subject(s) - trehalose , dextran , sucrose , desiccation , disaccharide , recombinant dna , amorphous solid , chemistry , dehydration , freeze drying , biochemistry , biology , gene , botany , chromatography , organic chemistry
Abstract The disaccharide trehalose is found to be effective for stabilization of active recombinant retroviruses in an amorphous dry state achieved through ambient‐temperature vacuum dehydration of retroviral supernatants. Studies revealed that trehalose is a significantly better desiccation protectant than sucrose, glucose, and dextran: dextran has essentially no protective effect on retroviral survival after drying and rehydration. X‐ray diffractometry of the retroviral supernatant dried with trehalose demonstrated its amorphous nature. The ability to dehydrate retroviral stocks at ambient temperatures into a stable glassy state will have a profound effect for researchers and commercial biotechnology companies which supply retroviral vectors for human gene therapy and basic research.