Premium
Decreasing Extracellular pH Increases CD13 Receptor Surface Content and Alters the Metabolism of HL60 Cells Cultured in Stirred Tank Bioreactors
Author(s) -
McDowell Christi L.,
Papoutsakis Eleftherios T.
Publication year - 1998
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp980050w
Subject(s) - hl60 , extracellular , flow cytometry , bioreactor , receptor , cell culture , metabolism , chemistry , biology , biochemistry , microbiology and biotechnology , cell , botany , genetics
Abstract Flow cytometry and Northern blotting were used to examine the effects of extracellular pH on CD13 receptor surface content and mRNA levels of HL60 (human promyelocytic leukemia) cells. Decreasing culture pH (7.4, 7.2, and 7.0) increased the CD13 receptor surface content of HL60 cells cultured at low agitation intensity (80 rpm) in 2‐L bioreactors. Unlike our earlier findings on the effects of increasing agitation rates and serum concentrations, changes in CD13 receptor content in response to decreasing culture pH did not correlate with changes in CD13 mRNA levels. Decreasing culture pH also decreased the average HL60 cell size. HL60 cells cultured at pH 7.0 and 7.2 exhibited glucose consumption and lactate production rates that were approximately 30−40% and 20−30% lower, respectively, than values of cells cultured at pH 7.4.