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Method for Construction of a Simple Laboratory‐Scale Nonwoven Filament Biocatalytic Filter
Author(s) -
Flickinger Michael C.,
Mullick Ashim,
Ollis David F.
Publication year - 1998
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp980049x
Subject(s) - materials science , polyester , silicone rubber , composite material , thread (computing) , polyamide , sealant , natural rubber , filter paper , chromatography , chemistry , computer science , operating system
A method is described for constructing a simple laboratory‐scale nonwoven filament biocatalytic filter (FBF) from multilayer acrylic vinyl acetate copolymer coated thread (yarn) potentially useful for investigation of biotransformations using entrapped enzymes or viable microorganisms. The porous structure of a commercial 128 μm, 279 denier, 100% polyester thread was sealed with a latex coat (precoat), then coated with a latex + sulfanilamide‐azocasein mixture, and finally coated with a latex top coat to generate the multilayer coated thread. The FBF was constructed by drawing the thread across a 4 cm diameter rubber O‐ring in the form of multiple layers and sealing the layers with silicone sealer. The FBF is combatible with a commercially available filter housing containing a flow distrtibutor. A method for characterizing the permeability of the sealant (top) coat of the FBF using azocasein release was also developed.