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Insertion of Stabilizing Loci in Vectors of T7 RNA Polymerase‐Mediated Escherichia coli Expression Systems: A Case Study on the Plasmids Involving Foreign Phospholipase D Gene
Author(s) -
Mishima Naoto,
Mizumoto Kousaku,
Iwasaki Yugo,
Nakano Hideo,
Yamane Tsuneo
Publication year - 1997
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp970084o
Subject(s) - plasmid , microbiology and biotechnology , recombinant dna , escherichia coli , t7 rna polymerase , biology , gene , plasmid preparation , gene expression , genetics , bacteriophage , pbr322
Plasmids carrying stabilizing loci were used in the expression of phospholipase D (PLD) gene fused with pel B signal sequence by a recombinant strain of Escherichia coli BL21 (DE3) using T7 RNA polymerase mediated expression system. By checking the living cell number and the percentage of the plasmid‐bearing cells, it was found that the plasmids involving PLD gene were not stable under noninduced conditions and that, after the induction, the number of plasmid‐bearing cells were rapidly decreased to almost zero. Then, a biologically stabilizing locus such as par B , ccd , or par was inserted into the plasmids. The newly constructed plasmids were maintained very stably in the recombinant cells until the cells were induced. However, after the induction, almost all the recombinant cells were rapidly killed due to highly toxic PLD. Using the best one of the stabilized PLD‐expressing plasmids, PLD production was improved 2‐fold.