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Bioreactor Headspace Purging Reduces Dissolved Carbon Dioxide Accumulation in Insect Cell Cultures and Enhances Cell Growth
Author(s) -
MitchellLogean Christine,
Murhammer David W.
Publication year - 1997
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp970078s
Subject(s) - bioreactor , carbon dioxide , chromatography , cell culture , cell , chemistry , oxygen , cell growth , population , doubling time , biology , botany , biochemistry , medicine , organic chemistry , genetics , environmental health
Flushing the bioreactor headspace of Sf‐9 and High Five insect cell cultures sparged with pure oxygen prevented the accumulation of CO 2 in the culture medium and enhanced the cell growth rate and maximum cell densities. The CO 2 accumulated to a concentration of ∼24 mM in both cultures in the absence of headspace purging compared to levels of 3 and 6 mM in the purged Sf‐9 and High Five cultures, respectively. Headspace purging decreased the mean population doubling times of the Sf‐9 and High Five cell lines from 38 to 22 h and from 46 to 22 h, respectively. In addition, headspace purging increased the maximum cell densities obtained in the Sf‐9 and High Five cell cultures from ∼(5–6) to ∼(8–10) × 10 6 cells/mL and from ∼(3–5) to ∼(8–10) × 10 6 cells/mL, respectively.