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Fast Ion‐Exchange HPLC of Proteins Using Porous Poly(glycidyl methacrylate‐ co ‐ethylene dimethacrylate) Monoliths Grafted with Poly(2‐acrylamido‐2‐methyl‐1‐propanesulfonic acid)
Author(s) -
Viklund Camilla,
Svec Frantisek,
Fréchet Jean M. J.,
Irgum Knut
Publication year - 1997
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp9700667
Subject(s) - glycidyl methacrylate , polymerization , methyl methacrylate , chemical engineering , monolithic hplc column , porosity , chemistry , polymer chemistry , chromatography , high performance liquid chromatography , ethylene , ion exchange , methacrylate , materials science , ion , polymer , organic chemistry , catalysis , engineering
Abstract Porous poly(glycidyl methacrylate‐ co ‐ethylene dimethacrylate) monoliths with different porous properties grafted with poly(2‐acrylamido‐2‐methyl‐1‐propanesulfonic acid) chains using cerium(IV) initiated free‐radical polymerization have been prepared and used for the separation of proteins in ion‐exchange HPLC mode. Because of the presence of the large pores that are typical of monolithic separation media which allow easy flow of all of the mobile phase, the efficiency of the columns does not deteriorate even at high flow velocities as a result of the specific morphology of the monoliths. Optimization of the chromatographic conditions such as the shape of the mobile phase gradient and the flow rate allows for very fast separation of three proteins in less than 1.5 min.