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Immobilization of Xylanase in Chitosan−Xanthan Hydrogels
Author(s) -
Dumitriu Severian,
Chornet Esteban
Publication year - 1997
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp970059i
Subject(s) - xylanase , chemistry , chitosan , enzyme , self healing hydrogels , immobilized enzyme , chromatography , biochemistry , polymer chemistry
endo ‐1,4‐β‐Xylanase (E.C. 3.2.1.8) has been immobilized in hydrogel beads prepared by complexation between chitosan and xanthan. The enzyme immobilization efficiency is between 88 and 98% within a wide range of concentrations of xylanase. The optimum xylanase activity requires a pH between 5.0 and 5.5. The immobilized enzymes show 60–70% higher activity than free enzymes. The K m of the immobilized xylanase increases with the concentration of the enzyme in the beads. The latter show, by electron microscopy, a predominantly fibrilar microstructure within which there are regions having globular shapes where the enzymes are lodged.