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Kinetic Parameters for Pressure−Temperature Inactivation of Bacillus subtilis α‐Amylase under Dynamic Conditions
Author(s) -
Ludikhuyze Linda R.,
Van den Broeck Ilse,
Weemaes Carla A.,
Hendrickx Marc E.
Publication year - 1997
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp970053t
Subject(s) - isobaric process , glycerol , bacillus subtilis , isothermal process , chemistry , kinetics , kinetic energy , thermodynamics , chromatography , biochemistry , biology , bacteria , physics , genetics , quantum mechanics
Abstract Pressure−temperature inactivation kinetics of two enzymatic model systems based on Bacillus subtilis α‐amylase (BSA), previously determined under isobaric−isothermal conditions, were studied under more realistic, i.e. dynamic, process conditions. The proposed kinetic model was found to accurately describe non‐isobaric/non‐isothermal inactivation of BSA in absence as well as in presence of 15% glycerol. Furthermore, the effect of pressure cycling on the inactivation of BSA (in absence of glycerol) was investigated. Multiple application of pressure seemed to exert a more pronounced inactivation effect as compared to a single‐cycle process of the same total time. This additional effect, however, could be completely attributed to the more extensive temperature variation occurring during pressure cycling.