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Comparison of Two Convection‐Aided Protein Adsorption Methods Using Porous Membranes and Perfusion Beads
Author(s) -
Kubota Noboru,
Konno Yoshitaka,
Miura Suguru,
Saito Kyoichi,
Sugita Kazuyuki,
Watanabe Kohei,
Sugo Takanobu
Publication year - 1996
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp960076s
Subject(s) - membrane , adsorption , chromatography , chemistry , ion exchange , porosity , polymer , perfusion , bovine serum albumin , albumin , chemical engineering , ion , organic chemistry , biochemistry , medicine , engineering , cardiology
We describe results which serve as a guide in the selection of protein recovery techniques using two new chromatographic methods based on anion‐exchange interaction, i.e., membrane and perfusion chromatographies, which involve enhancement of protein transport by convective flow. Bovine serum albumin solution was permeated through a functionalized porous hollow‐fiber membrane and a bed charged with functionalized porous (perfusion) beads of identical volume. The pores of the membrane are surrounded by grafted polymer chains immobilizing anion‐exchange groups, whereas the throughpores of the beads are surrounded by the diffusive pores at the periphery of which anion‐exchange groups are immobilized. An 8‐fold increase in throughput of the protein using the porous membrane, 37 mg of BSA/mL/min, was achieved at a low operating pressure of 0.1 MPa, compared to that using the perfusion bed.