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Characterization of the Chemoenzymatic Synthesis of N ‐Acetyl‐ d ‐neuraminic Acid (Neu5Ac)
Author(s) -
Blayer Simone,
Woodley John M.,
Lilly Malcolm D.,
Dawson Michael J.
Publication year - 1996
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp9600647
Subject(s) - neuraminic acid , biotransformation , epimer , chemistry , enzyme , aldolase a , biochemistry , stereochemistry , sialic acid
Abstract The characterization of the chemoenzymatic synthesis of N ‐acetyl‐ d ‐neuraminic acid using a base‐catalyzed epimerization and Neu5Ac aldolase‐catalyzed condensation is presented. The characterization has been used to exemplify a structured approach to biotransformation process design. In addition, the option of integration of the epimerization with the biotransformation (at alkaline pH) was considered. Process operating windows have been generated as a result of this approach, defining possible operating regions for subsequent scale‐up.