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Biodegradation of Hydrogen Sulfide by a Laboratory‐Scale Immobilized Pseudomonas putida CH11 Biofilter
Author(s) -
Chung YingChien,
Huang Chihpin,
Tseng ChingPing
Publication year - 1996
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp960058a
Subject(s) - pseudomonas putida , biofilter , biodegradation , hydrogen sulfide , pseudomonas , sulfide , chemistry , environmental chemistry , microbiology and biotechnology , environmental science , bacteria , biochemistry , sulfur , biology , organic chemistry , enzyme , environmental engineering , genetics
A heterotrophic Pseudomonas putida CH11 was isolated from livestock farming wastewater and applied for the treatment of H 2 S‐containing gas. Extensive tests including removal characteristics, metabolic products, and removal efficiencies of H 2 S by P . putida CH11 were examined in batch and continuous systems. The optimum pH required to remove hydrogen sulfide was found in the range of 6–8. The maximum removal rate and the saturation constant were calculated to be V m = 1.36 g S/day·kg dry bead and K s = 45.9 ppm, respectively. The main metabolic product of H 2 S oxidation was determined to be elemental sulfur. When P . putida CH11 was immobilized within Ca alginate, the cells exhibited high H 2 S removal efficiency, in excess of 96%, at concentrations of hydrogen sulfide from 10 to 150 ppm (flow rates of 36 and 72 L/h). These results suggest that P . putida CH11 immobilized within Ca alginate has the potential to be used as a H 2 S removal agent.