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Response Surface Methodology To Optimize β‐Galactosidase Immobilization Using a Combination of Hydrocolloids as the Supporting Matrix
Author(s) -
Mammarella Enrique J.,
Rubiolo Amelia C.
Publication year - 1996
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp9600546
Subject(s) - response surface methodology , sodium alginate , lactose , chemistry , viscosity , hydrolysis , matrix (chemical analysis) , chromatography , carrageenan , chemical engineering , sodium , materials science , composite material , food science , organic chemistry , engineering
Response surface methodology (RSM) was applied to optimize the composition of a mixture of hydrocolloids (low‐viscosity sodium alginate, high‐viscosity sodium alginate, and κ‐carrageenan) used in the immobilization of β‐galactosidase for application in the hydrolysis of lactose. A five levels, a three‐factor design was adopted. The activity and stability of the immobilized enzyme and the strength of the supporting matrix were optimized by using a mathematical model applied in the range of process conditions. Requirements for multifactor response surface designs were satisfied, and the correlation coefficient, R 2 , was larger than 0.850, ensuring a good adjustment of the model to the experimental values. Best results were obtained for values of 1.00% low‐viscosity sodium alginate, 1.40−1.60% high‐viscosity sodium alginate, 0.10−0.30% κ‐carrageenan, and 10−12% enzyme.