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Effects of Growth Rate on the Production of Pseudomonas fluorescens Lipase during the Fed‐Batch Cultivation of Escherichia coli
Author(s) -
Kim Seong Soo,
Kim Eun Ki,
Rhee Joon Shick
Publication year - 1996
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp960047h
Subject(s) - pseudomonas fluorescens , lipase , escherichia coli , food science , microbiology and biotechnology , pseudomonas , chemistry , biology , bacteria , biochemistry , enzyme , genetics , gene
Abstract Recombinant Escherichia coli was grown to produce the thermostable Pseudomonas fluorescens lipase by controlling the specific growth rate (μ). At low growth rate (μ = 0.07 h − 1 ), a higher cell density (OD 600 = 140) was obtained. By calculating the cell yield on nitrogen, the medium was optimized to prevent inhibition of cell growth by nitrogen unbalance. Acetate accumulated in the broth at the same rate as observed in a chemostat under nitrogen‐limited fed‐batch conditions, but not in a glucose‐limited fed‐batch culture. Postinduction growth rate affected lipase production more than preinduction growth rate. After induction, more lipase was produced at a low growth rate (μ = 0.1 h − 1 ) than at a high growth rate (μ = 0.4 h − 1 ). More lipase was produced at 37 than at 20 °C at a constant growth rate, indicating that temperature affected other factors in addition to growth rate.