Premium
Mass Transfer of Antibiotics Adsorbed by Human Serum Albumin in Hemodialyzers
Author(s) -
Kanamori Toshiyuki,
Takeshita Takaaki,
Sakai Kiyotaka
Publication year - 1996
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp960036obp217
Subject(s) - antibiotics , adsorption , chemistry , human serum albumin , dialysis , chromatography , ultrafiltration (renal) , langmuir adsorption model , mass transfer , serum albumin , albumin , drug , hemodialysis , pharmacology , medicine , surgery , biochemistry , organic chemistry
The removal rate of a drug by a hemodialyzer is very important to determine the dosage of the drug to a patient without kidney function. Mass transfer of a drug in a hemodialyzer and a patient's body is more complicated than that of ordinary solutes because most drugs are adsorbed by serum albumin. In this paper, we reveal the adsorption characteristics of five clinical antibiotics onto human serum albumin (HSA) with new experimental methods and discuss the influence of adsorption characteristics on removal of antibiotics by hemodialyzers using mathematical models. The adsorption equilibrium of the antibiotics with HSA followed the Langmuir isotherm. Adsorption rates of the antibiotics onto HSA were measured using a constant‐flow stirred tank reactor with an ultrafiltration module. A kinetic model for antibiotic transfer in a hemodialyzer and a patient's body was derived with the parameters obtained by the above experiments. Validity of the model was confirmed by dialysis experiments using hemodialyzers and antibiotic solutions including HSA. Removal estimation of a drug by hemodialysis therapy is feasible by the model with the parameters of the Langmuir isotherm for the drug.