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A Column Design for Reducing Viscous Fingering in Size Exclusion Chromatography
Author(s) -
Fernandez Erik J.,
Norton T. Tucker,
Jung William C.,
Tsavalas John G.
Publication year - 1996
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp960031r
Subject(s) - size exclusion chromatography , flow (mathematics) , viscous fingering , chromatography , instability , chemistry , mechanics , work (physics) , magnetic resonance imaging , materials science , physics , thermodynamics , medicine , biochemistry , radiology , porous medium , porosity , enzyme , organic chemistry
Under high concentration overload conditions, resolution during size exclusion chromatography of proteins can be compromised due to nonuniform flow caused by the viscous fingering flow instability. In this work, the nonuniform flow under these chromatographic conditions is analyzed by numerical simulation and magnetic resonance imaging. On the basis of an improved understanding of the flow phenomena, a new column design is proposed. The effectiveness of the design is evaluated by chromatography, magnetic resonance imaging, and numerical simulation.