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Primary Separation of the Entomopathogenic Products of Bacillus thuringiensis
Author(s) -
VillafañaRojas Juan,
Gutiérrez Eduardo,
de la Torre Mayra
Publication year - 1996
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp960030z
Subject(s) - bacillus thuringiensis , filtration (mathematics) , centrifuge , spore , chromatography , chemistry , centrifugation , stack (abstract data type) , biology , microbiology and biotechnology , bacteria , mathematics , statistics , genetics , physics , nuclear physics , computer science , programming language
An efficient recovery of the active spore−crystal complex of Bacillus thuringiensis was achieved by using either a disk‐stack centrifuge or a rotary vacuum filter. In both operations the spore recovery efficiency was higher than 99%. Nevertheless, the concentration of dry solids produced by each method strongly differed, being 31.5% for filtration and 7.5% for centrifugation, while the organic solids concentration increased 9.2 and 2.5 times, respectively. Adjustment of the broth to pH 4 improved the solid−liquid separation efficiency.
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