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Study of the Mass Transfer Kinetics of BSA on a TSK‐GEL DEAE‐5PW Anion Exchanger in a Wide Concentration Range
Author(s) -
GuanSajonz Hong,
Sajonz Peter,
Zhong Guoming,
Guiochon Georges
Publication year - 1996
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp960025v
Subject(s) - kinetics , chemistry , chromatography , mass transfer , adsorption , langmuir adsorption model , mass transfer coefficient , bovine serum albumin , volumetric flow rate , analytical chemistry (journal) , ion exchange , ion , thermodynamics , organic chemistry , physics , quantum mechanics
Abstract The adsorption isotherms of bovine serum albumin (BSA) on TOSOHAAS TSK‐GEL DEAE‐5PW were determined in a pH = 7.5 buffer for a 1.0 mL/min flow rate and at pH = 6.0 for 1.0, 2.0, and 3.0 mL/min flow rates. The isotherm data were derived from the breakthrough curves obtained by step series frontal analysis (FA) method. The sample concentrations ranged from ca. 0.2 to 3 mg/mL. From the Scatchard plots, it was found that the bi‐Langmuir isotherm model fits the adsorption isotherm data better than the single Langmuir isotherm model at both pH = 7.5 and 6.0, for flow rates up to 2.0 mL/min. The rate of the mass transfer kinetics was determined for each breakthrough curve by using a lumped kinetic model. An average rate coefficient of mass transfer kinetics was derived. This rate coefficient depends linearly on the average sample concentration.