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Characterization of the Stress Response of a Bioluminescent Biological Sensor in Batch and Continuous Cultures
Author(s) -
Rupani Sameer P.,
Gu Man Bock,
Konstantinov Konstantin B.,
Dhurjati Prasad S.,
Van Dyk Tina K.,
LaRossa Robert A.
Publication year - 1996
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp960015u
Subject(s) - bioluminescence , inducer , dilution , strain (injury) , escherichia coli , sos response , chemistry , heat shock , kinetics , biology , microbiology and biotechnology , chromatography , biochemistry , heat shock protein , gene , physics , anatomy , quantum mechanics , thermodynamics
The effects of temperature, growth stage, and inducer (ethanol) concentration on the kinetics and magnitude of the stress response were investigated by using an Escherichia coli strain with the grpE heat shock promoter fused to the Vibrio fischeri lux genes. When stressed, the cells responded by changing the level of specific light emission, which was measured both on‐ and off‐line. These measurements were used to characterize and optimize the sensitivity of the construct by determining the conditions at which the culture exhibited maximum specific bioluminescence and minimum response time to ethanol induction in batch cultivation. The results of the batch study were then applied to continuous cultivation, and the effect of dilution rate was determined. These results are of considerable interest in the development of an on‐line biological sensor system for the detection and toxicity assessment of chemical pollutants.