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Protein Separation via Polyelectrolyte Coacervation: Selectivity and Efficiency
Author(s) -
Wang Yingfan,
Gao Jeff Y.,
Dubin Paul L.
Publication year - 1996
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp960013+
Subject(s) - coacervate , polyelectrolyte , selectivity , chemistry , chromatography , chemical engineering , biochemistry , organic chemistry , polymer , catalysis , engineering
Selective phase separation with polyelectrolytes can be used to separate a mixture of proteins. The efficiency of separation was examined using the cationic polyelectrolyte poly(diallyldimethylammonium chloride) and the model proteins bovine serum albumin, β‐lactoglobulin, γ‐globulin, and ribonuclease A. The coacervation yield for individual proteins and the degree of separation, for selected protein pairs, were studied as a function of polymer molecular weight, ionic strength, and pH.
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