Premium
Optimization Study of Escherichia coli TB1 Cell Disruption for Cytochrome b 5 Recovery in a Small‐Scale Bead Mill
Author(s) -
Belo I.,
Santos J. A. L.,
Cabral J. M. S.,
Mota M.
Publication year - 1996
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp950085l
Subject(s) - escherichia coli , lysozyme , factorial experiment , response surface methodology , recombinant dna , bead , chemistry , chromatography , intracellular , cell disruption , cytochrome b5 , suspension (topology) , fractional factorial design , microbiology and biotechnology , enzyme , biochemistry , biology , cytochrome , materials science , mathematics , statistics , homotopy , pure mathematics , composite material , gene
The recovery of a recombinant intracellular protein, cytochrome b 5 , from Escherichia coli TB1 cells was carried out by bead mill disintegration in a discontinuous small‐scale instrument. This process was optimized by the use of experimental factorial design. Several parameters were studied: operating time, amount and size of beads, cellular suspension concentration, and presence of toluene and lysozyme. For the experimental conditions used, only the time of treatment and bead load had significant effects. The optimal values of these variables were found by applying the response surface methodology.