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Effects of Glycerol on β‐Lactamase Production during High Cell Density Cultivation of Recombinant Escherichia coli
Author(s) -
Kwon Soonjo,
Kim Sungsoo,
Kim Eunki
Publication year - 1996
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp9500728
Subject(s) - glycerol , escherichia coli , recombinant dna , lac operon , chemistry , enterobacteriaceae , isopropyl , microbiology and biotechnology , biochemistry , biology , gene , organic chemistry
Abstract Recombinant Escherichia coli was cultured up to OD 600 90 with glycerol fed by the DO‐STAT method to produce recombinant β‐lactamase. Optimum feeding and induction conditions were determined. When glycerol was maintained at a high concentration (40−50 g/L) after isopropyl thiogalactoside (IPTG) induction at OD 600 75, a 2‐fold increase in the amount of soluble β‐lactamase was obtained. Similarly, a 3‐fold increase was obtained by the addition of NaCl (0.4 M) at low glycerol concentration (10 g/L).