Premium
Local‐Equilibrium Behavior of Retained pH and Ionic Strength Gradients in Preparative Chromatography
Author(s) -
Frey Douglas D.
Publication year - 1996
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp950061e
Subject(s) - ionic strength , chemistry , chromatography , elution , ion exchange , ionic bonding , ion chromatography , base (topology) , ion , organic chemistry , mathematics , mathematical analysis , aqueous solution
A local‐equilibrium theory is developed and used to investigate the behavior of retained pH and ionic strength gradients formed on a strong‐base anion‐exchange column where both anion‐ and cation‐forming buffering species are present. Analytical relations are presented for several simplified cases of practical interest and are used to illustrate factors governing the selection of the elution and presaturation buffers when retained pH and ionic strength gradients are employed for preparative protein chromatography.