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Development of a Technique for Microimmobilization of Proteins on Silicon Wafers by a Streptavidin−Biotin Reaction
Author(s) -
Koyano Takeshi,
Saito Minoru,
Miyamoto Yasuo,
Kaifu Katsuaki,
Kato Masakazu
Publication year - 1996
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp9500573
Subject(s) - streptavidin , wafer , biotin , silicon , nanotechnology , chemistry , materials science , optoelectronics , biochemistry
In order to enable the future construction of molecular devices made of functional proteins, we have developed a technique for microimmobilization of proteins into organized patterns on a silicon wafer. A specific binding reaction between streptavidin and biotin forms the basis of our method. Active and inactive biotin regions were created on a silicon wafer using a photolithographic technique. Circuit‐type patterns and 0.75/1.5 μm line‐and‐space patterns of a model fluorescent protein, B‐phycoerythrin, were successfully created on a silicon wafer.