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Problems of Translating Heterologous Genes in Expression Systems: The Role of tRNA
Author(s) -
Smith David W. E.
Publication year - 1996
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp950056a
Subject(s) - heterologous , transfer rna , gene , heterologous expression , biology , translation (biology) , gene expression , genetics , messenger rna , rna , recombinant dna
tRNA can have large effects on the expression and overexpression of heterologous genes in microbial expression systems through reduced translation and errors in amino acid sequences of protein products. Examples are given of large effects on gene expression related to tRNA content and to tRNA base modifications, both of which differ in heterologous expression systems compared to the cells from which the genes originally came. tRNA should be of greater concern in the expression of heterologous genes.