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Phosphate Glass as a Phosphate Source in High Cell Density Escherichia coli Fermentations
Author(s) -
Curless Craig,
Baclaski Jeffrey,
Sachdev Raj
Publication year - 1996
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp9500369
Subject(s) - polyphosphate , phosphate , sodium , fermentation , sodium phosphates , escherichia coli , chemistry , sodium hexametaphosphate , phosphorus , food science , nuclear chemistry , biochemistry , organic chemistry , gene
Polyphosphate glass (sodium hexametaphosphate, sodium polyphosphate, glassy) was identified and tested as a source of phosphate that supports growth of recombinant Escherichia coli to cell densities over 110 g dry wt/L. Polyphosphate glasses cost no more than sodium and potassium phosphates, are more soluble than any other inorganic phosphate source, and do not form metallophosphate precipitates when mixed with other nutrients in proportions found in fermentation medias. Using a typical fermentation medium, 40% higher cell densities were obtained using polyphosphate glass rather than orthophosphate. Polyphosphate glasses will allow more flexibility in designing and executing high cell density fermentations.