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Enzymic Thin Film Coatings for Bioactive Materials
Author(s) -
Tong Xiaodong,
Trivedi Archana,
Jia Hongfei,
Zhang Minjuan,
Wang Ping
Publication year - 2008
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp0704135
Subject(s) - hydrolysis , aqueous solution , biofouling , protease , chemical engineering , thin film , coating , chemistry , materials science , immobilized enzyme , subtilisin , bovine serum albumin , membrane , chromatography , enzyme , organic chemistry , nanotechnology , biochemistry , engineering
Bioactive materials have been explored for a broad range of applications including biocatalysts, biosensors, antifouling membranes and other functional and smart materials. We report herein a unique method for preparation of bioactive materials through a spin coating process. Specifically, we investigated the preparation of protease Subtilisin Carlsberg‐coated plastic films and examined their activities for hydrolysis of chicken egg albumin (CEA). The process generated enzymic coatings with a typical loading of 13 μg/cm 2 , retaining 46% of the enzyme activity for hydrolysis of CEA in aqueous solutions. Interestingly, the surface‐coated protease thin film not only catalyzed the hydrolysis of CEA in aqueous solutions, but also showed good activity for solid‐state CEA that was coated on top of the enzyme thin film.