Premium
Conversion of Linoleic Acid into 10‐Hydroxy‐12( Z )‐octadecenoic Acid by Whole Cells of Stenotrophomonas nitritireducens
Author(s) -
Yu InSik,
Kim HyeJung,
Oh DeokKun
Publication year - 2008
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp0703073
Subject(s) - linoleic acid , yield (engineering) , substrate (aquarium) , chemistry , stenotrophomonas , bacteria , chromatography , biochemistry , food science , biology , fatty acid , pseudomonas , materials science , ecology , genetics , metallurgy
The conversion of linoleic acid into 10‐hydroxy‐12( Z )‐octadecenoic acid by whole cells of Stenotrophomonas nitritireducens as an isolated bacterium was optimized, and the optimal temperature, pH, and cell and substrate concentrations were 30 °C, 7.5, and 20 and 20 g/L, respectively. Under these conditions, whole cells in a bioreactor produced 15 g/L 10‐hydroxy‐12( Z )‐octadecenoic acid in 2 h of reaction time without detectable byproducts. Using 2 g/L linoleic acid, the cells produced 1.92 g/L 10‐hydroxy‐12( Z )‐octadecenoic acid. These are the highest concentration and yield of 10‐hydroxy‐12( Z )‐octadecenoic acid ever reported.
Accelerating Research
Robert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom
Address
John Eccles HouseRobert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom