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Conversion of Linoleic Acid into 10‐Hydroxy‐12( Z )‐octadecenoic Acid by Whole Cells of Stenotrophomonas nitritireducens
Author(s) -
Yu InSik,
Kim HyeJung,
Oh DeokKun
Publication year - 2008
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp0703073
Subject(s) - linoleic acid , yield (engineering) , substrate (aquarium) , chemistry , stenotrophomonas , bacteria , chromatography , biochemistry , food science , biology , fatty acid , pseudomonas , materials science , ecology , genetics , metallurgy
Abstract The conversion of linoleic acid into 10‐hydroxy‐12( Z )‐octadecenoic acid by whole cells of Stenotrophomonas nitritireducens as an isolated bacterium was optimized, and the optimal temperature, pH, and cell and substrate concentrations were 30 °C, 7.5, and 20 and 20 g/L, respectively. Under these conditions, whole cells in a bioreactor produced 15 g/L 10‐hydroxy‐12( Z )‐octadecenoic acid in 2 h of reaction time without detectable byproducts. Using 2 g/L linoleic acid, the cells produced 1.92 g/L 10‐hydroxy‐12( Z )‐octadecenoic acid. These are the highest concentration and yield of 10‐hydroxy‐12( Z )‐octadecenoic acid ever reported.