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FabG Mediates Polyhydroxyalkanoate Production from Both Related and Nonrelated Carbon Sources in Recombinant Escherichia coli LS5218
Author(s) -
Nomura Christopher T.,
Tanaka Tomoyo,
Eguen Tenai E.,
Appah Alexandria S.,
Matsumoto Ken'ichiro,
Taguchi Seiichi,
Ortiz C. Leo,
Doi Yoshiharu
Publication year - 2008
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp070303y
Subject(s) - polyhydroxyalkanoates , escherichia coli , monomer , chemistry , carbon source , recombinant dna , enzyme , biochemistry , carbon fibers , bacteria , gene , biology , polymer , organic chemistry , materials science , genetics , composite number , composite material
Polyhydroxyalkanoates (PHAs) composed of a mixture of short‐chain‐length‐medium‐chain‐length (SCL‐MCL) hydroxyacyl monomers are biologically produced polyesters that have properties ranging from thermoplastic to elastomeric, dependent on the molar ratio of SCL to MCL monomers incorporated into the copolymer. Because of the potential wide range of properties and applications for SCL‐MCL PHA copolymers, it is important to develop and characterize novel metabolic pathways for SCL‐MCL PHA production. The current study shows that coexpression of fabG genes from either E. coli or Pseudomonas sp. 61–3 with fabH (F87T) and PHA synthase genes enhances the production of SCL‐MCL PHA copolymer from both related and nonrelated carbon sources in Escherichia coli LS5218, indicating the flexibility of FabG as a monomer‐supplying enzyme for biological PHA production.