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Design and Construction of a Preparative‐Scale Dynamic Field Gradient Focusing Apparatus
Author(s) -
Tracy Noah I.,
Huang Zheng,
Ivory Cornelius F.
Publication year - 2008
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp070251c
Subject(s) - scale (ratio) , field (mathematics) , selection (genetic algorithm) , computer science , concentration gradient , scale up , process engineering , chemistry , chromatography , mathematics , physics , engineering , artificial intelligence , quantum mechanics , classical mechanics , pure mathematics
A linear model is used to show that dynamic field gradient focusing (DFGF) can be scaled to preparative capacity, ∼O (10 mgs). This paper explains how the preparative‐scale DFGF apparatus was designed and fabricated. Scaled‐down experiments and mathematical modeling guided material selection and design changes during construction to increase the probability that the prototype preparative‐scale DFGF apparatus would perform as intended. The finished prototype successfully focused bovine hemoglobin from an initial concentration of 6.82 to 15 mg/mL and allowed for 86% recovery of injected protein.