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Optimized Formation of Detergent Micelles of β‐Carotene and Retinal Production Using Recombinant Human β,β‐Carotene 15,15′‐Monooxygenase
Author(s) -
Kim NamHee,
Kim YeongSu,
Kim HyeJung,
Oh DeokKun
Publication year - 2008
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp070239k
Subject(s) - retinal , micelle , beta carotene , monooxygenase , chemistry , carotene , recombinant dna , chromatography , toluene , enzyme , pigment , aqueous solution , biochemistry , organic chemistry , carotenoid , cytochrome p450 , gene
The formation of β‐carotene detergent micelles and their conversion into retinal by recombinant human β,β‐carotene 15,15′‐monooxygenase was optimized under aqueous conditions. Toluene was the most hydrophobic among the organic solvents tested; thus, it was used to dissolve β‐carotene, which is a hydrophobic compound. Tween 80 was selected as the detergent because it supported the highest level of retinal production among all of the detergents tested. The maximum production of retinal was achieved in detergent micelles containing 200 mg/L of β‐carotene and 2.4% (w/v) Tween 80. Under these conditions, the recombinant enzyme produced 97 mg/L of retinal after 16 h with a conversion yield of 48.5% (w/w). The amount of retinal produced, which is the highest ever reported, is a result of the ability of our system to dissolve large amounts of β‐carotene.