Premium
Novel Orbital Shake Bioreactors for Transient Production of CHO Derived IgGs
Author(s) -
Stettler Matthieu,
Zhang Xiaowei,
Hacker David L.,
de Jesus Maria,
Wurm Florian M.
Publication year - 2007
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp070219i
Subject(s) - chinese hamster ovary cell , bioreactor , recombinant dna , cell culture , transfection , transient (computer programming) , hamster , yield (engineering) , biology , chemistry , microbiology and biotechnology , gene , biochemistry , materials science , genetics , computer science , botany , metallurgy , operating system
Large‐scale transient gene expression in mammalian cells is being developed for the rapid production of recombinant proteins for biochemical and preclinical studies. Here, the scalability of transient production of a recombinant human antibody in Chinese hamster ovary (CHO) cells was demonstrated in orbitally shaken disposable bioreactors at scales from 50 mL to 50 L. First, a small‐scale multiparameter approach was developed to optimize the poly(ethylenimine)‐mediated transfection in 50 mL shake tubes. This study confirmed the benefit, both in terms of extended cell culture viability and increased product yield, of mild hypothermic cultivation conditions for transient gene expression in CHO cells. Second, the scalability of the process was demonstrated in disposable shake bioreactors having nominal volumes of 5, 20, and 50 L with final antibody yields between 30 and 60 mg L −1 . Thus, the combination of transient gene expression with disposable shake bioreactors allows for rapid and cost‐effective production of recombinant proteins in CHO cells.