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Long‐Term Maintenance of a Transgenic Catharanthus roseus Hairy Root Line
Author(s) -
Peebles Christie A. M.,
Gibson Susan I.,
Shanks Jacqueline V.,
San KaYiu
Publication year - 2007
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp0702166
Subject(s) - catharanthus roseus , transgene , green fluorescent protein , biology , genetically modified crops , hairy root culture , cell culture , microbiology and biotechnology , botany , gene , biochemistry , genetics , agrobacterium
Stably transformed transgenic hairy root cultures have the potential to be a valuable production platform for a variety of secondary metabolites. This study reports that a transgenic hairy root culture of Catharanthus roseus has been stably maintained for over 4.5 years. This culture carries a transgene that expresses the green fluorescent protein under the control of the glucocorticoid‐inducible promoter. Genomic PCR confirmed the presence of the GFP insert within the hairy roots, and induction with dexamethasone caused a significant ( p < 0.02) increase in GFP levels.