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Separation of Murine Neutrophils and Macrophages by Thermoresponsive Magnetic Nanoparticles
Author(s) -
Hoshino Akiyoshi,
Ohnishi Noriyuki,
Yasuhara Masato,
Yamamoto Kenji,
Kondo Akihiko
Publication year - 2007
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp070185e
Subject(s) - magnetic nanoparticles , immunomagnetic separation , nanoparticle , conjugated system , magnetic separation , macrophage , materials science , immunoassay , chemistry , antibody , nanotechnology , biophysics , polymer , in vitro , chromatography , biochemistry , immunology , biology , metallurgy , composite material
Magnetic particles have been used widely in both biotechnological and medical fields, including for immunoassay, enzyme immobilization, drug transport, and immunological diagnosis. Especially particles with bioactive molecules such as antibodies and streptavidin are very useful tools for cell separation. Here we report affinity selection of neutrophils and macrophages from peritoneal inflammatory cells performed by thermoresponsive magnetic nanoparticles conjugated with macrophage‐specific anti‐F4/80 antibody. The magnetic nanoparticles, which are capped with thermoresponsive polymers, are aggregated by heating the particles over 30 °C and show their intrinsic magnetism. The neutrophils are concentrated approximately 90% by these magnetic nanoparticles without any activation, indicating that this novel cell separation method could fulfill a wide range of applications in analysis of the isolation of fragile cells such as neutrophils.