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A Study of the Interaction of HEK‐293 Cells with Streamline Chelating Adsorbent in Expanded Bed Operation
Author(s) -
Poulin Frédéric,
Jacquemart Renaud,
de Crescenzo Gregory,
Jolicoeur Mario,
Legros Robert
Publication year - 2008
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp070173t
Subject(s) - adsorption , chromatography , chemistry , hek 293 cells , centrifugation , expanded bed adsorption , chelation , filtration (mathematics) , chemical engineering , biochemistry , organic chemistry , engineering , gene , statistics , mathematics
Expanded bed adsorption (EBA) is an efficient protein purification process reducing time and steps of downstream processing (DSP) since nonclarified culture media can be processed directly without prior treatments such as filtration or centrifugation. However, cells and debris can interact with the adsorbent and affect bed stability as well as purification performance. To optimize EBA operating conditions these biomass/adsorbent interactions have to be understood and characterized. The adsorption of Human Embryonic Kidney cells (HEK 293) on unprimed and nickel‐primed metal affinity adsorbent was studied in a closed loop EBA setup. With the unprimed adsorbent, the overall level of interaction observed was nonsignificant. With the nickel‐primed adsorbent and an initial cell concentration ranging from 0.08 × 10 6 to 0.2 × 10 6 cells/mL, biomass/adsorbent interaction was found to be moderate and the adsorption apparent first‐order kinetic rate constant was determined to be k = 0.009 to 0.011 min ‐1 .