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Enhanced Vanillin Production from Recombinant E. coli Using NTG Mutagenesis and Adsorbent Resin
Author(s) -
Yoon SangHwal,
Lee EunGyeong,
Das Amitabha,
Lee SookHee,
Li Cui,
Ryu HeeKyoung,
Choi MyungSuk,
Seo WeonTaek,
Kim SeonWon
Publication year - 2007
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp070153r
Subject(s) - vanillin , ferulic acid , chemistry , mutagenesis , biochemistry , strain (injury) , escherichia coli , mutant , biology , gene , anatomy
Vanillin production was tested with different concentrations of added ferulic acid in E. coli harboring plasmid pTAHEF containing fcs (feruloyl‐CoA synthase) and ech (enoyl‐CoA hydratase/aldolase) genes cloned from Amycolatopsis sp. strain HR104. The maximum production of vanillin from E. coli DH5α harboring pTAHEF was found to be 1.0 g/L at 2.0 g/L of ferulic acid for 48 h of culture. To improve the vanillin production by reducing its toxicity, two approaches were followed: (1) generation of vanillin‐resistant mutant of NTG‐VR1 through NTG mutagenesis and (2) removal of toxic vanillin from the medium by XAD‐2 resin absorption. The vanillin production of NTG‐VR1 increased to three times at 5 g/L of ferulic acid when compared with its wild‐type strain. When 50% (w/v) of XAD‐2 resin was employed in culture with 10 g/L of ferulic acid, the vanillin production of NTG‐VR1 was 2.9 g/L, which was 2‐fold higher than that obtained with no use of the resin.