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Modeling Amino Acid Metabolism in Mammalian Cells‐Toward the Development of a Model Library
Author(s) -
Kontoravdi Cleo,
Wong Danny,
Lam Carolyn,
Lee Yih Yean,
Yap Miranda G. S.,
Pistikopoulos Efstratios N.,
Mantalaris Athanasios
Publication year - 2007
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp070106z
Subject(s) - amino acid , cell culture , metabolism , biochemistry , recombinant dna , protein biosynthesis , cell growth , hek 293 cells , cell metabolism , cell , biology , chinese hamster ovary cell , microbiology and biotechnology , computational biology , chemistry , genetics , gene
Amino acids are necessary to mammalian cell cultures both for protein synthesis and as an energy source. In this study, we present an unstructured mathematical model describing (i) cell growth and death kinetics and (ii) metabolism of glucose and 19 amino acids for HEK‐293 and CHO IFN‐γ cell cultures. The proposed mathematical framework is in good agreement with experimental data for both cell lines. It accommodates the inclusion of expressions for other cellular activities, such as the production of recombinant viral vectors or proteins, and can be used as the basis for the development of a model library for mammalian cell cultures.