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Carassius auratus ‐Originated Recombinant Histone H1 C‐Terminal Peptide as Gene Delivery Material
Author(s) -
Jung Hee Jung,
Hwang Dong Soo,
de Wei Quan,
Cha Hyung Joon
Publication year - 2008
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp070069b
Subject(s) - histone h1 , biology , histone , histone h4 , microbiology and biotechnology , recombinant dna , histone h2a , sap30 , gene , biochemistry
The effective delivery of exogenous genes into eukaryotic cells is important for fundamental and biotechnological research. Protein‐based gene delivery including histone proteins has recently emerged as a powerful technique for non‐viral DNA transfer. Histones are DNA‐binding proteins that function in DNA packaging and protection. In particular, histone H1 is largely responsible for the stabilization of higher‐order chromatin structures. Several studies have examined the use of full‐length histone H1‐mediated gene transfer, and a few studies have investigated the use of C‐terminal histone H1 fragments as gene‐transfer materials. Previously, we cloned a novel histone H1 cDNA from the goldfish Carassius auratus and found that a recombinant histone H1 C‐terminal short peptide (H1C) of 61 amino acids has comparable DNA binding and protection functions as full‐length histone H1. In the present work, we successfully expressed and purified soluble recombinant H1C in an Escherichia coli expression system using a hexahistidine tag fusion strategy and providing tRNAs for rare codons. We confirmed its DNA‐binding ability and found that this H1C peptide had similar or higher transfection efficiency in mammalian cells (human 293T and mouse NIH/3T3) than the widely used agent lipofectamine. Therefore, we suggest that this novel goldfish‐derived recombinant histone H1 C‐terminal short peptide could be used as a peptide‐based gene‐transfer mediator.