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Immobilization of Mesenchymal Stem Cells and Monocytes in Biocompatible Microcapsules to Cell Therapy
Author(s) -
Herrero Edgar P.,
Valle Eva M. Martín Del,
Galán Miguel A.
Publication year - 2007
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp070047p
Subject(s) - mesenchymal stem cell , biocompatible material , immune system , cell therapy , stem cell , chemistry , in vitro , membrane , microbiology and biotechnology , cell , regeneration (biology) , biophysics , biomedical engineering , immunology , medicine , biology , biochemistry
The aim of cell therapy is to replace, repair, or enhance the function of damaged tissues or organs. Several factors complicate the development of cellular therapies. Of primary importance is protection of the implanted cells from the hostapos;s immune system. Cells are encapsulated in selectively semipermeable and biocompatible membranes that block entry of immune mediators but allow outward diffusion of active molecules produced by the cells. The immobilization of mesenchymal stem cells and monocytes, in micrometric (30–60 μm) alginate‐barium microcapsules based on atomization processes, has been achieved successfully. This size is necessary to the administration of microcapsules via injection (Hamilton syringe with a needle size of 100 μm) and aerosol. Microencapsulated cells survive at least 2 weeks after preparation in vitro.