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Enhanced Hyaluronic Acid Production in Bacillus subtilis by Coexpressing Bacterial Hemoglobin
Author(s) -
Chien LiangJung,
Lee ChengKang
Publication year - 2007
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp070036w
Subject(s) - bacillus subtilis , gene , chemistry , escherichia coli , hyaluronic acid , biology , biochemistry , strain (injury) , hyaluronan synthase , biosynthesis , bacteria , microbiology and biotechnology , genetics , anatomy
Bacillus subtilis strains that can produce hyaluronic acid (HA) were constructed by integrating the HA synthase gene ( has A) and the UDP‐glucose dehydrogenase gene of group C Streptococcus ( has B) or of B. subtilis itself ( tau D) into the amy E locus of the B. subtilis chromosome. All of the inserted genes were under the control of a strong constitutive vegII promoter of B. subtilis . Although HA production could be achieved by expressing has A alone, coexpressing has B or tau D with has A could enhance HA production at least 2‐fold. To replenish the energy consumed for HA biosynthesis, Vitreoscilla hemoglobin (VHb) was coexpressed with the HA‐expressing genes. With the expression of VHb, not only the cell concentration was enhanced 25%, but also HA production was further increased by 100%. About 1.8 g/L of HA was obtained by the recombinant strain B. subtilis carrying VHb, has A, and tau D genes in the expression cassette after 30 h cultivation.